Cystatin C activity was determined using a quantitative ELISA assay kit (Elabscience Biotechnology Inc., Houston, TX, USA). The principle of assessment involves the quantitative sandwich enzyme immunoassay technique. This measurement was assessed in line with the instructions provided in the kit. The absorbance was measured spectrophotometrically at a wavelength of 450 nm. A standard curve with six distinct values ranging from 0.09 to 2.81 ng/mL was obtained for calculation. The results were expressed as ng/mL.
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