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Lipid oxidation was assessed using the thiobarbituric acid reactive substances (TBA-RS) method [40], using a standard curve of tetraethoxypropane.
Protein oxidation was evaluated by measuring the carbonyl groups formed during incubation with 2,4-dinitrophenylhydrazine (DNPH) in 2 N HCl [41].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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