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RNA-seq sample preparation

EM Emily Maclary
MH Michael Hinten
CH Clair Harris
SS Shriya Sethuraman
SG Srimonta Gayen
SK Sundeep Kalantry
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Total RNA was isolated from TRIzol (Life Technologies, #15596-018) according to the manufacturer’s instructions and submitted to the University of Michigan DNA Sequencing Core for Poly-A purification and strand-specific library preparation using the Illumina TruSeq Library preparation kit (Illumina, #RS-122-2103). Libraries were sequenced on the Illumina HiSeq2000 platform to generate 100 bp paired-end reads.

Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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