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IgG reformatting expression and purification
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The variable fragments corresponding to the heavy and light chain of the antibodies identified during the Sanger sequencing screening where subclined into a 2-vector system (one for the heavy chain and one for the light chain, expressed and purified as described in Leal et al. (manuscript under revision). For remaining antibodies, we outsourced at Genscript who supplied antibodies at 100 µg/mL concentration.
Copyright and License information: The Author(s) ©2023, corrected publication ©2024
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