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Immunostaining for IL-1B (Novus Biologicals, Cat NO: NB600-633)
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Immunostaining was performed according to the supplier's instructions (Novus Biologicals, Cat NO: NB600-633). Semi-quantification of antigen expression was evaluated under a light microscope at 40X magnification. Staining intensity was scored by two pathologists as follows: 0 indicated no staining, 1 indicated weak staining, 2 indicated moderate staining, 3 indicated strong staining, and 4 indicated very strong staining.
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