To generate a wild type UASp-CG31851 line, CG31851 coding regions (FlyBase ID: FBpp0080033) were cloned by PCR from genomic DNA into the pUASp vector (DGRC: Plasmid#1189) between the KpnI and XbaI restrictions sites. UAS lines were established through injection (Bestgene) using classical P-element mediated transformation.
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