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A protocol for the preparation of liposomes is described in detail elsewhere(43). Briefly, all liposomes used in these studies were extruded through a 100 nm filter and passed through a CL-4B column following extrusion. Liposomal composition consisted of 0.03% antigen (OVA), 1.5% CD22L, and 0.1% AF647, all of which were linked to PEG-DSPE according to previous reports (43, 50, 51). Primary human B-cells were isolated from healthy donor blood (The Scripps Research Institute normal blood donor service (NBDS).
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