CD8 T Cell Isolation and Subset Purification

PBMCs were thawed and rested overnight (O/N) in TexMacs medium. CD8 T cells were purified using the human REAlease CD8 MicroBead Kit. Naïve (CD45RA⁺CCR7⁺ or CD45RA⁺CD28⁺), EM (CD45RA⁻CCR7⁻ or CD45RA⁻CD28⁺), and TEMRA (CD45RA⁺CCR7⁻ or CD45RA⁺CD28⁻) CD3⁺CD8⁺ cells were sorted by FACS (FACSAria; BD Biosciences, Le Pont de Claix, France; purity >95%).