Human megakaryocytic cell line culture

JP Jessica E. Pritchard
JP Juliette E. Pearce
IS Inge A.M. Snoeren
SF Stijn N.R. Fuchs
KG Katrin Götz
FP Fabian Peisker
SW Silke Wagner
AB Adam Benabid
NL Niklas Lutterbach
VK Vanessa Klöker
JN James S. Nagai
MH Monica T. Hannani
AG Anna K. Galyga
ES Ellen Sistemich
BB Bella Banjanin
NF Niclas Flosdorf
EB Eric Bindels
KO Kathrin Olschok
KB Katharina Biaesch
NC Nicolas Chatain
NB Neha Bhagwat
AD Andrew Dunbar
RS Rita Sarkis
ON Olaia Naveiras
MB Marie-Luise Berres
SK Steffen Koschmieder
RL Ross L. Levine
IC Ivan G. Costa
HG Hélène F.E. Gleitz
RK Rafael Kramann
RS Rebekka K. Schneider
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MEG-01 cells were maintained in 90% RPMI 1640 (Gibco, #21875-091), 10% FCS and 1% Pen/Strep at a seeding density of 5.0 x 105/mL. SET-2 cells were maintained in 80% RPMI 1640, 20% FCS and 1% Pen/Strep at a seeding density of 8.0 x 105/mL. Cells were collected and processed for RNA isolation as described below.

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