MEG-01 cells were maintained in 90% RPMI 1640 (Gibco, #21875-091), 10% FCS and 1% Pen/Strep at a seeding density of 5.0 x 105/mL. SET-2 cells were maintained in 80% RPMI 1640, 20% FCS and 1% Pen/Strep at a seeding density of 8.0 x 105/mL. Cells were collected and processed for RNA isolation as described below.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.