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Immunofluorescence, histology and immunohistochemistry
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Immunofluorescence analysis of cells were performed as described previously [74] and analyzed using a Zeiss LSM710 confocal microscope with a Zeiss AxioObserver Z1 stand. Formalin-fixed paraffin-embedded (FFPE) tumors were sectioned with a thickness of 5 µm and stained for respective antigens as described previously [58]. Antigen retrieval was performed in citrate buffer with a pressure cooker. Breast cancer tissue microarray slides (TMA BC01) were purchased from Reveal Biosciences.
Copyright and License information: ©2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
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