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The immunofluorescence staining images were obtained as previously described [15]. The transfected HEK293T cells was probed by CaV1.2α1C antibody (Alomone Labs), and imaged with a Leica TCS SP5 confocal laser scanning microscopy (Leica Microsystems, Heidelberg, Germany). The detailed methods for immunofluorescence staining are given in the supplementary information S1 File.
Copyright and License information: ©2017 Liu et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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