Choline chloride (98%), lactic acid (85%), catechin hydrate (≥98%), epicatechin (≥98%), caffeine (≥98%), theobromine (≥98%), 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), sodium acetate (≥99%), glacial acetic acid, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), and iron(III) chloride hexahydrate, ethylene glycol, absolute alcohol (≥99.5%), and gallic acid (99.9%) were purchased Merck KGaA (Darmstadt, Germany). Glycerol (99.5%), anhydrous sodium carbonate (99.5%), and methanol (99.8%) were purchased from Loba Chemie Pvt. Ltd. (Mumbai, India), and Folin-Ciocalteu reagent from Winkler (Santiago, Chile).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.