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2.4 Flow cytometry assay

YD Yuexin Dong
XH Xinyang Hu
SX Shixin Xie
YS Yuxian Song
YH Yijia He
WJ Wanyong Jin
YN Yanhong Ni
ZW Zhiyong Wang
LD Liang Ding
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For the cell subtypes of PBMC analysis, cells were collected and washed with PBS twice and then suspended in 200 μL PBS. For enumeration of mature human T (CD3+) cells, helper/inducer T (CD3+ CD4+) cells, cytotoxic T (CD3+ CD8+) cells, B (CD19+) cells, and NK (CD3−CD16+and/orCD56+) lymphocytes, CD3−FITC/CD8−PE/CD45−PerCP/CD4−APC reagent, BD Multitest CD3−FITC/CD16−PE+ CD56−PE/CD45−PerCP/CD19−APC reagent were used according to the manufacturer’s instructions, respectively (Cat No.340503, BD Multitest™), then quantified by flow cytometry on a FACS Calibur instrument.

Copyright and License information:  ©2023 Dong, Hu, Xie, Song, He, Jin, Ni, Wang and Ding.
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