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Cell Segregation from the Peritoneal Cavity
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Peritoneal cells were garnered by washing the peritoneal cavity. In short, the peritoneal cavities of the mice were injected with 5 mL of ice-cold PBS, and then gently massaged to extract loosely attached cells. The PLF was drawn and washed twice with PBS by centrifugation at 300 × g for 10 min at 4 °C. The resulting pellet was suspended in 1 mL of ice-cold PBS.
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