Bone turnover markers

Souad Daamouch; Sylvia Thiele; Lorenz Hofbauer; Martina Rauner

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Bone turnover markers

SD Souad Daamouch

ST Sylvia Thiele

LH Lorenz Hofbauer

MR Martina Rauner

This method is extracted from research article: Endocr Connect, Sep 2023

Effects of adipocyte-specific Dkk1 deletion on bone homeostasis and obesity-induced bone loss in male mice

DOI: 10.1530/EC-23-0251

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After blood collection via heart puncture, serum was centrifuged for 10 min at 400 g . Serological analysis of bone turnover markers procollagen type 1 amino-terminal-propeptide (P1NP), C-terminal telopeptide of type I collagen (CTX) and tartrate-resistant acid phosphatase 5b (TRAcP 5b) were measured using commercially available ELISA kits from IDS (Frankfurt/Main, Germany). Dkk1 was measured using an ELISA kit from R&D Systems.

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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