Metabolomics samples of three times 10,000 animals per condition per triplicate were harvested immediately after 120 min of swimming exercise. Nematodes were freeze‐dried, homogenized, and processed separately for each triplicate for GC–MS analysis on a 7890A gas chromatograph equipped with a 7693 automatic sampler coupled to a 5975C mass single‐quadrupole detector (Agilent). See Supplemental Methods (section ‘Metabolomics’) for a detailed protocol description.
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