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When the cells were full in the 12-well plates, we used a micropipette tip to draw lines along the central axis of the wells and then added the culture medium to the wells after PBS washing. We observed the migration distance of cells and calculated the cell migration rate at 0 h and 48 h.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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