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Total RNA was isolated from cultured cells, dissociated lung and subcutaneous tumor tissues using QiaAmp RNA Blood kit (Qiagen). cDNA templates were synthesized using Transcriptor First Strand cDNA Synthesis Kit (Roche) followed by polymerase chain amplification using GoTaq Flexi DNA Polymerase (Promega). Sequences of primers used for amplification will be provided upon request.
Copyright and License information: ©2016 Kansal et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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