Mouse peritonitis–sepsis infection model

A total of 48 female BALB/c mice were intraperitoneally injected with PBS or 1 g/kg PS for 5 d and then intraperitoneally infected with E. coli G92 suspension at a dose of 5.0 × 10⁸ CFUs. After 1 h of infection, the mice were randomly divided into control and antibiotic treatment groups, with 12 mice in each group. The control group received a single intraperitoneal dose of PBS, while the antibiotic treatment group received ciprofloxacin at a dosage of 25 mg/kg. The survival of the mice was monitored for a period of 5 d. The liver and kidney were aseptically removed and separated into 2 parts for CFU estimation or hematoxylin and eosin staining.