Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Blood samples, Hb measurement, and criteria for anemia

ZW Zheng Wen
JL Jianxiong Long
LZ Lulu Zhu
SL Shun Liu
XZ Xiaoyun Zeng
DH Dongping Huang
XQ Xiaoqiang Qiu
LS Li Su
request Request a Protocol
ask Ask a question
Favorite

After an overnight fast for at least 8 h, 5 mL of peripheral whole venous blood samples were collected in ethylene diamine tetra-acetic acid (EDTA) tubes for routine blood testing. Hb, mean corpuscular volume (MCV), and mean corpuscular hemoglobin concentration (MCHC) were measured by using an the automatic modular blood and body fluid analyzer (Sysmex NX 10 [B4], Japan). Hb cutoff concentrations for the diagnosis of anemia were < 13.0 g/dL for men and < 12.0 g/dL for non-pregnant women, according to the WHO recommendations [45]. Anemia type was further categorized according to MCV and MCHC measurements. Microcytosis, normocytosis, and macrocytosis were defined as MCV < 80 fL, MCV = 80–100 fL, and MCV > 100 fL, respectively. Hypochromia, normochromia, and hyperchromia were defined as an MCHC < 32 g/dL, MCHC = 32–36 g/dL, and MCHC > 36 g/dL, respectively [46].

Copyright and License information: The Author(s) ©2023
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A