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The right upper lobes of the lungs were fixed in 4% paraformaldehyde overnight and then embedded in paraffin. Briefly, we used myeloperoxidase (MPO, NBP1‐51148; Novus) and citrullinated histone H3 (ab5103; Abcam) antibodies as primary antibodies with Alexa Fluor 647‐conjugated anti‐rabbit and Alexa Fluor 488‐conjugated anti‐mouse secondary antibodies. DAPI (Thermo Fisher Scientific) was used as a nuclear counterstain. Images were captured using confocal microscopy (Leica SP8).
Copyright and License information: ©2023 The Authors. published by John Wiley & Sons Ltd on behalf of Pulmonary Vascular Research Institute.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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