Luciferase-reporter assay

YF Ying Fang
MZ Mu-Chen Zhang
YH Yang He
CL Chen Li
HF Hai Fang
PX Peng-Peng Xu
SC Shu Cheng
YZ Yan Zhao
YF Yan Feng
QL Qian Liu
LW Li Wang
WZ Wei-Li Zhao
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The promoter region (−1500/ + 24) of the SUV39H1 gene was PCR-amplified from human genome DNA. The primer sequences were as follows: forward primer: 5’-CCTGAGCTCGCTAGCCTCGAGGAACATCTAACTGAATGACAGCCT-3’; reverse primer: 5’-CAGTACCGGATTGCCAAGCTTTCCCCACGGCTAACGACA −3’. The amplified 1.5 kb fragment was then cloned into pGL4.14 vector between XhoI and HindIII sites. The resulting plasmid was confirmed by Sanger sequencing. Luciferase-reporter assay was conducted using Duo-Lite Luciferase Assay System (DD1205-01, Vazyme, Nanjing, China) according to the manufacturer’s protocols.

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