Sandfly captures.

Entomological captures (2020–2021) used HP light traps¹⁷ were performed under a license for zoological material collection (SISBio #15237-2) (Figure 2A). Systematic captures occurred in January, March, May, July, September, and November. Those areas were chosen based on the highest number of ATL cases. In 2020, 20 collection points (P1–P20) were in Córrego Volta Grande (Patrocínio de Caratinga district) (Figure 2B). In 2021, 10 collection points were selected, six (U1–U6) in the urban area of Caratinga (Figure 2C), and four in the district of Sapucaia (T7–T10) (Figure 2D). Insects were stored in glycerin before identification and molecular approaches.

Collection points of sand fly in municipality of Caratinga (A), Patrocínio de Caratinga district (B), urban area of Caratinga (C), and Sapucaia district (D).