Lysosome labeling with LysoTracker Red DND-99

DL Dan Liu
RX Ruinan Xing
QZ Quanyu Zhang
XT Xiaoxiang Tian
YQ Yanping Qi
HS Haixu Song
YL Yanxia Liu
HY Haibo Yu
XZ Xiaolin Zhang
QJ Quanmin Jing
CY Chenghui Yan
YH Yaling Han
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NMCMs were differentially stimulated and stained with LysoTracker Red DND-99 (300 nM, Thermo Fisher Scientific, L-7582) for 10 min according to the manufacturer’s protocol. The excess LysoTracker was removed with culture medium. The cells were fixed and permeabilized. Coverslips were mounted using ProLong Gold antifade reagent (Thermo Fisher Scientific). Images were captured using a fluorescence microscope at 40× magnification (Zeiss) and analyzed using Image-Pro Plus v6.0 software.

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