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Y-maze spontaneous alternation
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The Y-maze consisted of three identical arms at 120° angles from one other. Each mouse was placed in the centre of the maze and allowed to explore freely for 8 min. The apparatus was cleaned with 70% ethanol between animals. Trials were videotaped and analysed by Ethovision XT software, with the total number of arm entries, as well as the sequence of entries, calculated to obtain the percentage of alternations (defined as entries into a different arm than the one previously explored).38
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