Synergy analysis

The web-based application SynergyFinder 3.0 (https://synergyfinder.fimm.fi/) was used to analyze multidrug synergies (34). Data were uploaded as appropriately formatted tables and % inhibition was selected as a readout. Four-parameter logistic regression (LL4) curve fitting was applied and automated outlier detection was toggled “on”. The zero interaction potency reference model was used for synergy scoring and the correction parameter was toggled “on”. For Synergy scores greater than 10, drug interactions were considered to be synergistic. For synergy scores from 2 to 10 drug interactions were considered to be additive with scores from 9 to 10 approaching synergy.