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Human Peripheral Blood Mononuclear Cell (PBMC) Collection
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We measured the expression of Ezh2 in human peripheral Th cells using flow cytometry in both the allergic patients and the non-allergic controls. PBMCs were isolated from peripheral venous blood by Ficoll-Plaque plus (GE Healthcare, Uppsala, Sweden) density gradient centrifugation.
Copyright and License information: ©2017 Hou, Chen, Chou, Kan, Tsai and Cha.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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