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4.1. Plant Materials and DNA Extraction

PP Parama Praphasanobol
PP Putut Rakhmad Purnama
SJ Supaporn Junbuathong
SC Somsong Chotechuen
PM Peerapon Moung-Ngam
WK Waraluk Kasettranan
CP Chanita Paliyavuth
LC Luca Comai
MP Monnat Pongpanich
TB Teerapong Buaboocha
SC Supachitra Chadchawan
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The rice population used in this study comprised 230 rice varieties (Supplementary Table S1) grown at the Pathum Thani Rice Research Center, Rice Department, Ministry of Agriculture. The experiment used a completely randomized design with five replicates. This experiment was conducted during in-season rice cultivation from August to December 2017. DNA was extracted from young leaf tissue using the Genomic DNA Mini Kit (Plant; Geneaid Biotech Ltd., Taiwan) according to the manufacturer’s protocol. Genomic DNA (gDNA) was quantified using a NanodropTM spectrophotometer and confirmed using agarose gel electrophoresis. Genomic DNA was sequenced using a Genome Analyzer (Illumina, Inc., San Diego, CA, USA).

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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