2.7. Biochemical Evaluation

With the help of negative-pressure tubes containing a clot activator, 10 mLs of blood was collected and kept at room temperature for 15 min, then centrifuged for 10 min at 1300× g. The separated serum was pipetted and used for serum biochemical evaluation. Serum activities of ALP, ALT, AST, LDH, GGT, CK, and serum concentrations of urea, creatinine, and TP were evaluated. We conducted the analyses by utilizing a spectrophotometer (Labquest^® Bio 2000, Barueri, SP, Brazil), that is, semi-automatic and commercial colorimetric reagents (Kits Labtest^® Lagoa Santa, MG, Brazil). All blood samples were obtained through jugular venipuncture.