The melon (Cucumis melo) genotypes used in this study were the Korean accession PI 161375, cultivar Songwhan Charmi, the Spanish type Piel de Sapo, and the NIL SC12-1-99, which carries an introgression of SC on the linkage group XII that contains the cmv1 gene [8]. Seeds were pre-germinated and grown as described by Guiu-Aragonés et al. [11].
A clonal population of the aphid Myzus persicae, designated MP89 and originally from the ICA-CSIC (Madrid, Spain), was maintained on tobacco plants (Nicotiana tabacum ‘Xanthi’).
The cucumovirus strain CMV-LS was provided by Professor P. Palukaitis from Seoul’s Women’s University (Korea) as infectious clones of the three genomic RNAs [24]. The potyviruses ZYMV-AGII (attenuated strain) and watermelon mosaic virus LL2B3 (WMV-LL2B3) infectious clones were provided by Prof. A. Gal-On from Volcani Center (Israel) [25] and Dr. C. Desbiez from INRAE (France) [26], respectively.
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