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Electrical stimulation

RG Rachel M. Gwynne
KL Kenny D. K. N. Ly
LP Laura J. Parry
JB Joel C. Bornstein
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A unipolar stimulating electrode (50 μm stainless steel insulated with 15 μm Teflon) was used to deliver single pulses (1–2 mA, duration 0.5 ms, Master-8 stimulator, ISO-flex stimulus isolation unit, AMPI, Jerusalem, Israel) to the mucosa close to the location where chemical stimuli were applied. This was to test whether an intact neural pathway was present from that part of the mucosa to the recording site. Also, a comparison between the effects of antagonists on chemical vs. electrical stimuli was used to help identify the location(s) of receptors involved in the reflex pathway(s) activated. The mean latencies and amplitudes of IJPs evoked by electrical stimuli were calculated. Controls for stimulus spread have been conducted previously (Gwynne and Bornstein, 2007).

Copyright and License information:  ©2017 Gwynne, Ly, Parry and Bornstein.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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