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2.1.1. antimiR-33 Sponge Design and Composition
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The antimiR-33 sponge sequence was designed using the Kluiver method, specifically the oligonucleotide duplex approach [31], with two perfect binding sites for miR-33a; according to miRBase (5′-GUGCAUUGUAGUUGCAUUGCA-3′), each binding site is the antisense sequence of miR-33a. The 5′ and 3′ ends of the oligonucleotide duplex, corresponding to the antimiR-33 sponge, consist of overhangs that are compatible with the SacI and XmaI restriction enzymes, respectively.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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