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2.3.9. Negative Geotaxis (Climbing Assay)

SS Shabnam Shabir
AS Amit Sehgal
JD Joydeep Dutta
ID Inderpal Devgon
SS Sandeep K. Singh
WA Walaa F. Alsanie
AA Abdulhakeem S. Alamri
MA Majid Alhomrani
AA Abdulaziz Alsharif
MB Mohammed Abubaker Mohammed Basalamah
HF Hani Faidah
FB Farkad Bantun
AS Abdullah Ali Saati
EV Emanuel Vamanu
MS Mahendra P. Singh
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Anaesthetized test flies were kept in a vertical glass vial that was approximately 25 cm in length and 1.5 cm in diameter [52]. The flies were firmly tapped to the bottom of the vial after a short rest period. After one minute, flies that climbed to the top of the vial and those that stayed at the base were counted differently. Every 60 s, the data were expressed as a percentage of flies that eluded capture beyond a minimal distance of 6 cm. Each replication of the experiment used twenty adults. “The experiment was performed in triplicate”, and the average of the three trials for each fly group, including the control, was used to determine the findings for each replication. For each trial, a PI (performance index) was computed and presented as follows:

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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