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2.2. Construction of BEV, BCoV, and BRV standard plasmids

JC Junzhen Chen
DL Dan Li
YX Yafang Xu
ZL Zeyu Li
SM Siqi Ma
XL Xinyi Liu
YY Yuanyuan Yuan
CZ Chengyuan Zhang
QF Qiang Fu
HS Huijun Shi
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According to the GenBank database, combined with the published sequences of BEV-E, BCoV Npro, and the BRV-VP6 gene, the sequences were sent to Sangon Bioengineering (Shanghai) Co., Ltd. BEV, BCoV, and BRV PCR were cloned into pMD18-T, pGEM-T Easy Vector, and pUC18 vectors (purchased from ADDGENG), sequenced for validation, and mapped (Supplementary Figure S1). The specific PCR reaction system is shown in Supplementary Table S1. Reaction conditions were 95°C, 5 min; 95°C, 30 s; annealing, 30 s; 72°C, 1 min; 34 cycles; and 72°C, 10 min.

Copyright and License information:  ©2023 Chen, Li, Xu, Li, Ma, Liu, Yuan, Zhang, Fu and Shi.
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