Dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence probe ROS detection kit

HT-29 and SW620 cells were seeded in 12-well cell culture plates (1x10⁴ cells/well), cultured for 24 h and then treated with CuB (5 µM) in a 37˚C cell incubator for 48 h. The original culture medium was aspirated and DCFH-DA working solution (MedChemExpress) was added at a final concentration of 10 µM in a 37˚C incubator was 30 min, and the cells were observed and imaged under a fluorescence microscope. The average fluorescence intensity of each image was determined using ImageJ 1.53t software.