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To investigate the ability of IL-4 in inducing the expression of Foxp3 molecules on the CD4+ T cells of EW-fed R23-3 mice, R23-3 mice were injected intravenously with anti-IL-4 mAb (1 mg; clone 11B11) or control antibody (1 mg; rat IgG, Cappel, Cochranville, PA, USA) on the day before and on day 3 of receiving the diet. Mice were weighed daily for 7 days. On day 7, the mice were euthanized, and their MLN and spleen cells and intestinal tissues were harvested. The expression of Foxp3 molecules on splenic and MLN CD4+ T cells was analyzed by flow cytometry.
Copyright and License information: ©2017 Nakajima-Adachi et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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