The robust tissue-cultured ‘White heaven’ seedlings with the same size were selected for gene expression analysis. For HS, lily plantlets were incubated at 37 °C for different lengths of time (0, 0.5, 1, 3, 6, 12 h). After HS finished, the leaves were collected for extracting total RNA. The RNA reverse transcribed into cDNA with a HiScript II kit (Vazyme, China), and the expression of LlC3H18 were detected by real-time quantitative PCR (RT-qPCR) with the 2−ΔΔCT method (Livak and Schmittgen 2001; Schmittgen and Livak 2008). Lily 18S rRNA was used as an internal reference gene (Table S4).
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