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Gene annotation

YL Yafang Liu
BC Bin Chen
XL Xinyan Lu
SL Shuang Liu
DJ Dandan Jiang
XW Xuan Wang
LY Lin Yi
RL Rongyu Li
QZ Quanfu Zhang
LW Lixian Wu
XY Xing Yang
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Sequencing employed next-generation sequencing technology (NGS) on the Illumina NovaSeq platform. Annotations were facilitated via the MITOS WebServer (http://mitos.bioinf.uni-leipzig.de/index.py), with the A5-miseq v20150522 program utilized for the construction of the complete mitochondrial genome (14, 15). Alignment with closely related species in the NCBI database were performed to ascertain the location of protein-coding, tRNA, and rRNA genes. Predictive analyses of tRNA genes secondary structure were conducted on the tRNAscan-SE (http://lowelab.ucsc.edu/tRNAscan-SE/) online platform (16), and the CGView Server (https://paulstothard.github.io/cgview/) was employed for mapping mitochondrial genome circles. Analytical tools included DNAStar V7.1 for nucleotide composition analysis and CodonW 1.4.2 the relative synonymous codon usage (RSCU) computation.

Copyright and License information:  ©2023 Liu, Chen, Lu, Liu, Jiang, Wang, Yi, Li, Zhang, Wu and Yang.
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