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Quantification of intestinal barrier function biomarkers

FM Franz Martín
MB Manuel Blanco-Suárez
PZ Paola Zambrano
OC Oscar Cáceres
MA Miriam Almirall
JA José Alegre-Martín
BL Beatriz Lobo
AG Ana Maria González-Castro
JS Javier Santos
JD Joan Carles Domingo
JJ Joanna Jurek
JC Jesús Castro-Marrero
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Plasma ZO-1 (Catalog #: MBS706368, MyBioSource, San Diego, CA), LPS (Catalog #: MBS266722, MyBioSource, San Diego, CA), sCD14 (Catalog #: RK01060, Abyntek Biopharma, Vizcaya, Spain), and IL-1β (Catalog #: MBS2510385, MyBioSource, San Diego, CA) were measured using commercially available ELISA kits according to the manufacturers’ instructions. Circulating human anti-β-lactoglobulin antibody levels (IgG isotypes) were assayed using a validated home-made ELISA protocol as detailed below. All the plasma samples were measured in blind duplicates for each biomarker. For all protocols, absorbance at 450 nm with 570 nm correction was measured in a microplate reader, and corrected absorbance was interpolated in each standard curve to determine the concentrations (Sigma Plot).

Copyright and License information:  ©2023 Martín, Blanco-Suárez, Zambrano, Cáceres, Almirall, Alegre-Martín, Lobo, González-Castro, Santos, Domingo, Jurek and Castro-Marrero
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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