These were performed with hematoxylin and eosin procedures.25 Briefly, organs such as liver, spleen, kidney, and testes from each animal were fixed in 10% formal saline, grossed and cut longitudinally into pieces of 4 mm in thickness, and processed for histologic study using standard procedure. Microtome sections were dried on a hot plate and later stained with hematoxylin and eosin stains for examination with a light microscope.
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