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2.3. Determination of vitamin E concentration in plasma
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Plasma vitamin E concentrations were measured by high-performance liquid chromatography (HPLC) with fluorescence detection using an external standard of alpha-tocopherol (Sigma, United States) according to analytical methods described previously (34, 35).
Lipid-standardized vitamin E values were calculated as the ratio between the plasma vitamin E concentration (μmol/L) and the sum of the concentrations of total cholesterol (mmol/L) and triglycerides (mmol/L) in serum (36–38).
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