3.7. Verification of absence of random genomic plasmid integration

PCR amplification with KAPA2G Hotstart Genotyping Mix (Roche, #KK7352) was performed on plasmid DNA and genomic DNA extracted by DNeasy Blood & Tissue Kit (Qiagen, #69504) with the following parameters: 95 °C × 3 min, 30 cycles (98 °C × 20 s, 60 °C × 15 s, 72 °C × 20 s), 72 °C ×5 min, and 4 °C hold. The PCR products were visualized by agarose gel electrophoresis.