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Bacterial culture and isolation

FX Feiyun Xu
HL Hanpeng Liao
JY Jinyong Yang
YZ Yingjiao Zhang
PY Peng Yu
YC Yiying Cao
JF Ju Fang
SC Shu Chen
LL Liang Li
LS Leyun Sun
CD Chongxuan Du
KW Ke Wang
XD Xiaolin Dang
ZF Zhiwei Feng
YC Yifan Cao
YL Ying Li
JZ Jianhua Zhang
WX Weifeng Xu
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To isolate the putative strains from rhizosheath soil, fresh soil was suspended in diluent (NaCl 4.25 g L−1, KH2PO4 0.15 g L−1, Na2HPO4 0.3 g L−1, MgSO4 0.1 g L−1, gelatin 0.05 g L−1), and then plated on 0.5× TSA (0.5× TSA; 7.5 g L−1 tryptone, 2.5 g L−1 soytone, 2.5 g L−1 sodium chloride, and 15 g L−1 agar, pH 7.0)80. After incubation for 48 h at 30 °C, colonies were randomly isolated from the plates. A total of 224 strains were isolated, 113 and 111 from the acid and alkaline rhizosheath, respectively (Supplementary Table 2). Bacterial colonies were identified at the species level by sequencing 16 S rRNA gene using the primers 27 F and 1492 R (Supplementary Table 4). Next, we aligned the sequence reads using BLASTn, and identified the closest match.

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