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Gelatin zymography for MMP-9 analysis

JD Jie Deng
LG Lorne M. Golub
HL Hsi-Ming Lee
HB Heta-Dinesh Bhatt
FJ Francis Johnson
TX Tian-min Xu
YG Ying Gu
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After 18-h incubation, the conditioned media from cultured peritoneal Mφ were collected and analyzed for pro- (92 kDa) and active- (82 kDa) MMP-9 by gelatin zymography as described previously32, 33, 50, 51. The clear zones of lysis against a background indicated gelatinolytic activity and were scanned densitometrically with Invitrogen™ iBright™ FL1000 Imaging Systems (Thermo Fisher Scientific, Inc., Boston, MA, USA). Results were analyzed by Image J to quantitatively assess gelatinase activity31. MMP-9 standard was purchased from R&D Systems, Inc. (Minneapolis, MN, USA).

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