2.7. Blood Samples Were Taken from Mice to Detect Blood Lipids

There are 24 C57 mice (male) aged 6-8 weeks, of which 12 mice were fed with high fat and 12 mice were fed with normal. After 6 weeks, blood samples were collected from the eye socket of mice and placed in heparin anticoagulant tubes for 2 hours. After that, the samples were separated at 3000 rpm/heart for 15 min at 2-8°C. The thawed samples were centrifuged again and then tested for triglyceride, total cholesterol, HDL cholesterol, and LDL cholesterol levels.