An entomological investigation was carried out to collect fleas infesting domestic animals (dogs, cats, goats and sheep) in 22 sites located in Tunisia’s five bioclimatic zones (humid, sub-humid, semi-arid, arid and Saharan, Fig. 1). Individual fleas were placed in labeled flasks and stored in 70% ethanol. All specimens were identified to species level using the standard taxonomic keys [14]. A distribution map of collected fleas, according to the bioclimatic areas, was made using ArcGIS 9 software (version 9.3.1).
Map of Tunisia showing the distribution of flea collection sites and repartition of Bartonella infected fleas according to bioclimatic areas
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