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TEM was used to determine the morphology of the chosen VLF-loaded LPs.31 A small amount of LPs was applied to the carbon-coated paraffin sheet and allowed to sit for 1 minute after being diluted in an appropriate solvent. The grid was prepared and dried at 37 °C and set with a drop of (0.2 % w/v) phosphotungstate for 10 seconds after TEM analyzed the sample.
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