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Analysis of soil DNA viruses

QB Qicheng Bei
TR Thomas Reitz
BS Beatrix Schnabel
NE Nico Eisenhauer
MS Martin Schädler
FB François Buscot
AH Anna Heintz-Buschart
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To identify potential DNA virus sequences, VirSorter2 v2.2.3 [42] and CheckV v1.0.1 [43] were applied to contigs >5 kb. Only contigs with a VirSorter2 score ≥ 0.9, containing at least one hallmark gene, and at least one viral gene from CheckV were retained and clustered. The viral operational taxonomic units (vOTUs) and MAGs were further linked via clustered regularly interspaced short palindromic repeats (CRISPR) spacers (BLASTn, query coverage ≥ 95% and ≤1 mismatch) and shared genomic content (ANI ≥ 90% with 5000 bp). Detailed viral analysis can be found in the Supplementary Methods.

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