Total RNA was extracted from MDA–MB–231 cells treated with IgG antibody or rhPeriostin, and libraries were constructed using the VAHTS Stranded mRNA–seq Library Prep Kit for Illumina v2. The quality of the library was checked using Qubit 2100, and the concentrations were determined according to the analysis profiles. Genes expressed at a log2 (fold change) > 1 or < − 1 and a P < 0.05 were identified as differentially expressed genes and then subjected to Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses using DAVID Bioinformatic Resources 6.8.
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