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For mouse vaccination, groups of 6-8 weeks old female BALB/c mice were immunized intramuscularly with LNP-Z-mRNA_RBD (15 μg, n = 6), LNP-m1ψ-mRNA_RBD (15 μg, n = 6), or Placebo (phosphate-buffered saline (PBS), only, n = 6) in 100 μL volume using sterile BD U-100 insulin syringes. The sera of immunized mice were collected at indicated time points in Figure 4D. At 5 weeks after second immunization, mice were sacrificed and splenocytes were isolated to detect cellular immune responses by ELISPOT as described below.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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